TB-500 Dosage CalculatorTB-500 Reconstitution Calculator & Units Chart
TB-500 amounts are written in milligrams, not micrograms, so draws get large fast. Enter your vial, the bacteriostatic water and the amount your clinician specified to get the exact syringe units — plus a volume-comfort check, leftover-per-vial math, a BPC-157 blend mode and a 28-day expiry planner.
- ✔ mg → units for U-100, U-40, 1 mL & 3 mL syringes
- ✔ TB-500 + BPC-157 blend mode
- ✔ Over-1 mL injection alert
- ✔ Sourced to FDA & WADA
TB-500 is not an FDA-approved drug, and no human dose has been established. An FDA advisory committee voted in July 2026 to recommend it for the pharmacy-compounding list, but the FDA has not made a final decision. Thymosin β4 and its derivatives, including TB-500, are prohibited in sport at all times (WADA S2). This page does arithmetic on numbers you enter — it does not suggest or endorse any dose. Discuss any use with a licensed clinician.
TB-500 Units Calculator
Pick your vial, enter the water and the amount per injection. Every result, alert and chart updates as you type.
= 0.800 mL of a 2.5 mg/mL TB-500 solution · lands exactly on a printed mark
What each injection contains
blend breakdown| Peptide | In vial | Per injection | Concentration |
|---|
Label-accuracy check
same draw, real contentUnregulated vials aren’t tested to pharmacy standards, and some products sold as “TB-500” contain a different molecule. If the content differs from the label, the same reading delivers a different amount.
Supply planner
for your planned lengthCounts assume each vial is discarded 28 days after mixing, even if it isn’t empty.
28-day vial timeline
each block = 1 dayBAC water optimizer
same vial & amount, different water| Water | Concentration | Your draw | Readability |
|---|
Your vial label
How Many Units Is 2 mg of TB-500?
In a 5 mg TB-500 vial mixed with 2 mL of bacteriostatic water, 2 mg is 80 units on a U-100 insulin syringe (0.80 mL). In a 10 mg vial with 2 mL it is 40 units, and in a 5 mg vial with 1 mL it is also 40 units. The reading always depends on the water you added, so check your own vial in the calculator above.
Here is the arithmetic. 5 mg dissolved in 2 mL gives 2.5 mg — or 2,500 mcg — in every millilitre. A U-100 syringe splits a millilitre into 100 units, so each unit holds 25 mcg, and 2,000 mcg needs 80 units. Because TB-500 amounts are usually written in milligrams, readings are often ten times larger than for a microgram peptide such as BPC-157, and a single draw can approach a full 1 mL syringe.
TB-500 Reconstitution Chart (U-100 Units)
Syringe readings for common TB-500 vial sizes and water volumes. The amounts across the top are round numbers chosen to show the arithmetic, not dosing guidance.
| Vial + BAC water | Concentration | 0.5 mg | 1 mg | 2 mg | 2.5 mg |
|---|---|---|---|---|---|
| 2 mg + 1 mL | 2 mg/mL | 25 u | 50 u | 100 u | exceeds vial |
| 5 mg + 1 mL | 5 mg/mL | 10 u | 20 u | 40 u | 50 u |
| 5 mg + 2 mL | 2.5 mg/mL | 20 u | 40 u | 80 u | 100 u |
| 5 mg + 2.5 mL | 2 mg/mL | 25 u | 50 u | 100 u | 125 u · 2 draws |
| 5 mg + 3 mL | 1.67 mg/mL | 30 u | 60 u | 120 u · 2 draws | 150 u · 2 draws |
| 10 mg + 1 mL | 10 mg/mL | 5 u | 10 u | 20 u | 25 u |
| 10 mg + 2 mL | 5 mg/mL | 10 u | 20 u | 40 u | 50 u |
| 10 mg + 3 mL | 3.33 mg/mL | 15 u | 30 u | 60 u | 75 u |
| 20 mg + 2 mL | 10 mg/mL | 5 u | 10 u | 20 u | 25 u |
“u” = units on a U-100 insulin syringe (100 units = 1 mL). Anything over 100 units needs a larger syringe or two separate draws.
How many doses are in a TB-500 vial?
Because TB-500 amounts are measured in milligrams, a vial runs out quickly — and an amount that doesn’t divide evenly into the vial leaves an awkward remainder. The right-hand column shows a 2× weekly schedule against the 28-day discard window.
| Vial size | At 1 mg | At 2 mg | At 2.5 mg | 2× weekly at 1 mg |
|---|---|---|---|---|
| 2 mg | 2 | 1 | 0 (0.8) | Empty in ~4 days |
| 5 mg | 5 | 2 + 1 mg left | 2 | Empty in ~2 weeks |
| 10 mg | 10 | 5 | 4 | 2 doses past day 28 |
| 20 mg | 20 | 10 | 8 | 12 doses past day 28 |
How the TB-500 Reconstitution Math Works
Concentration (mg/mL) = vial mg ÷ water mL
Volume (mL) = amount mg ÷ concentration
Units = volume × 100 (U-100) or × 40 (U-40)
Doses per vial = vial mg ÷ amount mg (round down)
Worked example (the calculator’s default)
- 5 mg vial + 2 mL water → 5 ÷ 2 = 2.5 mg/mL
- 2 mg ÷ 2.5 = 0.80 mL → × 100 = 80 units
- 5 ÷ 2 = 2 full doses + 1 mg left over — about one week at 2× weekly
Less water, smaller draws
With microgram peptides, people add more water to make tiny doses readable. TB-500 is the opposite: with milligram amounts, extra water pushes draws over 1 mL. The same 5 mg vial with 1 mL of water halves the 2 mg draw to 40 units. The optimizer in the calculator flags which volumes keep you inside one syringe.
Pharmacist’s note: injection volume
Subcutaneous injections are generally kept to about 1 mL per site, because larger volumes tend to sting and leak back. If the calculator says a draw is over 1 mL, treat that as a prompt to talk to your clinician about the mixing volume or splitting the dose — not as an instruction to inject more.
TB-500 vs Thymosin Beta-4: Not the Same Molecule
“TB-500” and “thymosin beta-4” are often used as if they were interchangeable. They aren’t, and the difference matters for both the evidence and the label on your vial.
| Thymosin beta-4 (Tβ4) | TB-500 (as usually sold) | |
|---|---|---|
| What it is | A natural 43-amino-acid protein found in most human cells | A synthetic, N-acetylated 7-amino-acid fragment (Ac-LKKTETQ), matching positions 17–23 of Tβ4 |
| Human research | Early-phase trials of eye drops, topical gel and an IV safety study | No published human clinical trials |
| FDA view (July 2026) | Separate substance | Briefing noted naming and preparation ambiguities and no human clinical evidence for the fragment |
| Sport (WADA 2026) | Prohibited, S2 | Named explicitly as a prohibited derivative, S2 |
Because the name is applied loosely, a vial labelled “TB-500” may contain the fragment, full-length Tβ4 or something else. Only mass-spectrometry identity testing — not purity testing alone — can confirm which.
Why This Page Has No “Loading Phase” or Body-Weight Calculator
“Loading” and “maintenance” come from forums
The loading-then-maintenance schedules repeated across the web were never tested in a human trial of TB-500. Building them into a calculator would present guesswork as protocol.
Most of the evidence is for a different molecule
The animal and early human studies people cite mostly used full-length thymosin β4. A 2024 laboratory study even suggested the TB-500 fragment itself may not drive wound healing, while one of its breakdown products might.
Weight-based scaling isn’t a dose
Converting animal mg/kg figures to people is a method for choosing a cautious first-in-human trial starting dose, not a treatment dose. This tool converts the amount your clinician gives you — nothing more.
What Research on TB-500 and Thymosin Beta-4 Exists
| Research | Molecule | What it can and can’t tell you |
|---|---|---|
| Rat wound-healing study (Malinda et al., 1999) | Full-length Tβ4 | Faster wound closure in animals; the classic citation behind TB-500 marketing. |
| IV safety study in healthy volunteers | Full-length Tβ4 | Phase 1 tolerability data; not an efficacy study and not TB-500. |
| Topical gel trials for skin ulcers (phase 2) | Full-length Tβ4 | Small trials; no approved product resulted. |
| Eye-drop trials for dry eye and corneal disease | Full-length Tβ4 | The most developed human program; still not FDA-approved. |
| Laboratory study of the fragment (2024) | TB-500 (Ac-LKKTETQ) | Reported the fragment did not enhance wound healing in that model; a metabolite did. |
| Human trials of TB-500 | TB-500 | None published |
Is TB-500 Legal? FDA and WADA Status in 2026
TB-500 is not FDA-approved. The live question is whether a licensed pharmacy may compound it with a prescription.
- 2023
FDA lists the thymosin beta-4 fragment (LKKTETQ) in Category 2 — bulk substances that raise significant safety concerns — restricting compounding.
- February–April 2026
HHS announces a review of the restricted peptide list, and FDA signals its intent to remove a group of peptides, including those later sent to its advisory committee, from Category 2.
- July 23, 2026
FDA’s Pharmacy Compounding Advisory Committee reportedly votes 8–6 (one abstention) to recommend TB-500 for the 503A bulks list for wound healing. FDA staff had recommended against listing, citing naming ambiguity and no human clinical evidence.
- As of October 16, 2026
We found no final FDA rule. Committee votes are advisory; listing still requires proposed rulemaking, public comment and a final decision.
Banned in sport — no exemption
WADA’s 2026 Prohibited List names “thymosin-β4 and its derivatives, e.g. TB-500” under S2 growth factors, prohibited in and out of competition. Athletes are strictly liable for anything found in a sample, and TB-500 also shows up in horse-racing doping cases.
“Research use only” means untested for people
Vials labelled for research are not made, tested or sterilized to pharmaceutical standards. Independent testing of unregulated peptides has found endotoxins, truncated sequences and the wrong molecule — the reason for this page’s label-accuracy check.
TB-500 Safety, Storage and Mixing Basics
What is known about safety
- There are no peer-reviewed human safety studies of TB-500 itself.
- FDA has warned that peptide products can trigger immune reactions when they contain aggregates or impurities.
- Thymosin β4 is involved in cell migration and blood-vessel growth; whether that matters for people with a current or past cancer is unknown.
- No data exist for pregnancy, breastfeeding or drug interactions.
- A non-sterile product can cause infection regardless of the peptide.
Reconstitution and storage
- Bacteriostatic water (0.9% benzyl alcohol) allows repeat entry; sterile water has no preservative and is single-use.
- Run the water down the inside of the vial and swirl gently — don’t shake.
- Refrigerate the mixed vial at 2–8 °C, away from light; don’t freeze it.
- Under USP <797>, a preserved multiple-dose vial generally has a 28-day beyond-use date after first puncture.
Questions to Ask Your Doctor or Pharmacist
- Is there an approved treatment for my injury or condition with stronger evidence?
- Does this product have an independent mass-spectrometry identity test, not only an HPLC purity result?
- Is the product the TB-500 fragment or full-length thymosin β4 — and does that change anything?
- Do I have a cancer history or another reason to avoid a growth-related peptide?
- What volume should I mix to, and can you check my first syringe reading with me?
- Am I subject to sports, military or workplace drug testing?
Related Peptide Calculators
This TB-500 tool is part of the DexoCalc peptide cluster. Use the universal calculator for any other compound, or a dedicated tool when your peptide has its own vial sizes or schedule.
ALLPeptide Calculator — Reconstitution & Dosage
The universal tool for any peptide, vial or syringe, including three-peptide blends.
BPC-157 Dosage Calculator
TB-500’s most common blend partner. Use it when your amount is set in mcg of BPC-157.
Open calculator → Not FDA-approvedGHK-Cu Dosage Calculator
The copper peptide in GLOW blends with TB-500. Adds a copper-per-injection check.
Open calculator → Not FDA-approvedCJC-1295 / Ipamorelin Calculator
Another two-peptide vial; shows how much of each peptide you get per unit.
Open calculator → FDA-approved (Egrifta)Tesamorelin Dosage Calculator
An approved, mg-dosed peptide with labelled reconstitution steps — a useful benchmark.
Open calculator → FDA-approved RxSemaglutide Reconstitution Calculator
Weekly mg-based GLP-1 math for compounded vials.
Open calculator → FDA-approved RxTirzepatide Dosage Calculator
Maps labelled titration steps to syringe units.
Open calculator → InvestigationalRetatrutide Dosage Calculator
Arithmetic for an investigational weekly peptide still in trials.
Open calculator →TB-500 Dosage Calculator FAQs
In a 5 mg vial with 2 mL of bacteriostatic water, 2 mg is 80 units on a U-100 syringe. In a 10 mg vial with 2 mL, or a 5 mg vial with 1 mL, it is 40 units.
In a 5 mg vial with 2 mL it is 100 units — a full 1 mL syringe. In a 10 mg vial with 2 mL it is 50 units.
1 mL gives 5 mg/mL and 2 mL gives 2.5 mg/mL. Because TB-500 amounts are in milligrams, less water keeps draws smaller. The optimizer shows which volumes keep your prescribed amount within one syringe.
Adding 2 mL of bacteriostatic water gives 5 mg/mL, so each U-100 unit holds 50 mcg. Run the water slowly down the vial wall, swirl gently and refrigerate. At small amounts a 10 mg vial may not be used within 28 days.
There isn’t one. TB-500 has no published human clinical trials, and the “loading” and “maintenance” figures online come from forums. This calculator converts an amount your clinician specifies; it does not recommend one.
No. Thymosin beta-4 is a 43-amino-acid protein. TB-500 usually refers to a synthetic 7-amino-acid fragment of it (Ac-LKKTETQ). Most research people cite used the full protein, not the fragment.
It is not FDA-approved. An FDA advisory committee voted in July 2026 to recommend adding it to the 503A compounding list for wound healing, but as of October 2026 the FDA had not issued a final rule.
Yes. WADA’s 2026 Prohibited List names thymosin-β4 and its derivatives, including TB-500, under S2. It is prohibited at all times.
Use the TB + BPC Blend mode. Enter the mg of each peptide in the vial; the units follow your TB-500 amount and the table shows how much BPC-157 comes with it. If you dose by BPC-157, use the BPC-157 calculator instead.
TB-500 amounts are usually in milligrams, so a dilute mix can push a single draw past 1 mL. Less water concentrates the solution and shrinks the volume. Ask your clinician before changing your mixing volume.
Stability data for unregulated vials are limited. A preserved multiple-dose vial is generally given a 28-day beyond-use date after first puncture under USP <797>. Keep it refrigerated.
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Manzoor Ahmad, Pharm-D
Licensed Pharmacist · Founder, DexoCalc
Manzoor is a licensed pharmacist with more than nine years of experience writing about supplements and medication safety, and 13+ years building web tools. He runs a community pharmacy, where checking dilution arithmetic is routine work. He built this calculator, verified every chart value by hand, and reviews the regulatory section whenever the FDA or WADA position changes. He does not sell peptides and has no affiliate relationships with peptide vendors.
Sources
- U.S. FDA. Bulk Drug Substances Used in Compounding.
- U.S. FDA. Certain Bulk Drug Substances for Use in Compounding that May Present Significant Safety Risks.
- Buchanan Ingersoll & Rooney. FDA voted yes on six peptides — now what? (2026).
- AJMC. 5 things to know about the FDA’s peptide reversal, August 21, 2026.
- World Anti-Doping Agency. 2026 Prohibited List (S2 growth factors).
- Malinda KM, et al. Thymosin β4 accelerates wound healing. J Invest Dermatol. 1999. PubMed 10469335.
- BSCG. TB-500 peptide: benefits, safety, legal status and research.
- U.S. FDA. Estimating the Maximum Safe Starting Dose in Initial Clinical Trials (2005 guidance).
- U.S. Pharmacopeia. General Chapter <797>.
Disclaimer: This TB-500 calculator is an educational arithmetic tool. TB-500 is not approved by the FDA or any major regulator for human use, and nothing on this page recommends, endorses or provides a dose, product, source or protocol. DexoCalc does not sell peptides and has no affiliate relationships with peptide vendors. Consult a licensed clinician and pharmacist before using any unapproved substance.